منابع مشابه
Detection of multiple mycoplasma infection in cell cultures by PCR.
A total of 301 cell cultures from 15 laboratories were monitored for mycoplasma (Mollicutes) using PCR and culture methodology. The infection was detected in the cell culture collection of 12 laboratories. PCR for Mollicutes detected these bacteria in 93 (30.9%) samples. Although the infection was confirmed by culture for 69 (22.9%) samples, PCR with generic primers did not detect the infection...
متن کاملDetection of mycoplasma contamination in cell cultures and bovine sera
Contamination of cell cultures and sera used for animal virus propagation with mycoplasmas represents a serious problem, especially in virology. Therefore specific control measures must be used. To achieve this we introduced PCR for the detection of mycoplasma species in cell cultures and compared its results with ELISA and microbiological culture. Seven mycoplasma species which are the most co...
متن کاملMycoplasma Infection of Cell Cultures
This book is a collection of papers dealing with one of the most ubiquitous and troublesome nuisances of present day research in cell biology. Mycoplasma contamination of cell lines in a cell culture laboratory can cause changes in growth and biochemical properties of the cells, thus introducing uncontrolled variables into the experimental system. The papers in this book were presented at a wor...
متن کاملDetection of mycoplasma contamination in cell cultures by a mycoplasma group-specific PCR.
The suitability of a 16S rRNA-based mycoplasma group-specific PCR for the detection of mycoplasma contamination in cell cultures was investigated. A total of 104 cell cultures were tested by using microbiological culture, DNA fluorochrome staining, DNA-rRNA hybridization, and PCR techniques. A comparison of the results obtained with these techniques revealed agreement for 95 cell cultures. Disc...
متن کاملElimination of Mycoplasma Contamination in Cell Cultures
These antibiotic treatments had a high efficiency of permanent cure: MRA 64%, ciprofloxacin 77%, BM-Cyclin 84%. Resistance to mycoplasma eradication was observed in some cell cultures: BM-Cyclin 5%, ciprofloxacin 14%, MRA 22%. Nearly all resistant contaminants that could be identified belonged to the species M. arginini and M. orale. Detrimental effects of the antibiotics were seen in form of c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Journal of Pathology and Bacteriology
سال: 1967
ISSN: 0368-3494,1555-2039
DOI: 10.1002/path.1700930112